I was wondering if you had any experience with running perMANOVA’s in R using the package vegan (adonis function)?
I am working with several explanatory variables and many clinical biochemistry/hematological response variables (all continuous, non-normally distributed).
My explanatory variables are: ID (29 levels), Treatment (2 levels), Sex (2 levels) and Exposure/Time (11 levels) – repeat exposure
I am interested in (1) whether the response variable changes over time with repeated exposure and (2) whether the 2 treatment groups are significantly different from each other and whether this effect varies with time.
I think I understand how to calculate the within subject (ID) variation: